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Figure 4 | Molecular Cancer

Figure 4

From: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway

Figure 4

DIF-1 suppressed VEGF-induced angiogenesis in Matrigel plug. VEGF-containing Matrigel was injected subcutaneously into the flanks of 6-week old C57/BL6 mice. Seven days later, Matrigel plug was extracted and embedded in paraffin. Sections were stained with hematoxylin-eosin staining (A-D) and immunofluorescence staining using PECAM-1(CD31) (E-H) (Higher magnification of the boxed areas in A, C, E and G are shown in B, D, F and H, respectively). The scale bars indicate 500 μm. (I) The expression of PECAM-1 (CD31) was analyzed with fluorescence microscopy and expressed as the strength of fluorescence in Matrigel-plug (mean ± SE) of three independent experiments. The asterisk indicates *P < 0.05 versus control.

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Molecular Cancer

ISSN: 1476-4598