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Figure 6 | Molecular Cancer

Figure 6

From: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway

Figure 6

The effects of DIF-1 on VEGF receptors, VEGFR-1 and VEGFR-2. (A) DIF-1 decreased VEGFR-1 and VEGFR-2 expression in HUVECs. HUVECs were incubated with or without DIF-1 (30 μM) for the periods indicated and the samples were subjected to Western blot analysis for VEGFR-1 and VEGFR-2. The levels of protein bands were quantified and are shown as percentage of the control level at time 0. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.05 **P < 0.001 versus control. (B) DIF-1 did not affect phosphorylation level of VEGFR-2. HUVECs were incubated with or without DIF-1 (30 μM) for the periods indicated and the samples were subjected to Western blot analysis using anti-phospho-VEGFR-2 (Tyr1175) antibody. The membrane was re-probed with anti-VEGFR-2 antibody. The levels of protein bands were quantified and are shown as percentage of the control level at time 0. Values are mean ± SE of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598