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Figure 3 | Molecular Cancer

Figure 3

From: Differential expression of DKK-1 binding receptors on stromal cells and myeloma cells results in their distinct response to secreted DKK-1 in myeloma

Figure 3

Effects of MM cells on the mRNA expressions of LRP5/6, Krm1/2 and β-catenin in SCs from healthy donors in coculture with RPMI-8226. A1: SCs from healthy donors. A2: SCs from healthy donors in coculture with RPMI-8226 in the absence of a Transwell insert. A3: SCs from healthy donors in coculture with RPMI-8226 in the presence of a Transwell insert. LRP5/6, Krm1/2 and β-catenin gene expressions were detected in SCs from healthy donors after 24h-72 h coculture with RPMI-8226 in the presence or absence of a Transwell insert. No significant effect on the gene expression was observed after 24 h coculture. After 48 h, a significant up-regulation of LRP5(fold change, 3.5), LRP6 (fold change, 4), Krm1(fold change, 4) and Krm2 (fold change,5) together with an down-regulation of β-catenin (fold change, - 1.3) in SCs from healthy donors were seen whether in the presence or absence of a Transwell insert. After 72 h, mRNA expression of LRP5(fold change, 7), LRP6 (fold change, 5), Krm1(fold change, 7) and Krm2 (fold change, 4.5) were elevated, meanwhile mRNA expression of β-catenin (fold change, -2.7) was decreased.

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Molecular Cancer

ISSN: 1476-4598