Figure 1

Induction and removal of cisplatin-induced DNA lesions. A) Clonogenic cell survival curves of 833 K and SuSa testis tumour cells and MGH-U1 bladder cancer cells after cisplatin treatment for 1 h. B) MGH-U1 bladder cancer cells were treated with cisplatin (7.5 or 15 μg/ml) for 1 h, DNA was isolated 0 h, 6 h or 24 h post-treatment and examined for the presence of GpG-intrastrand crosslinks. C) MGH-U1 bladder cancer cells, 833 K and SuSa testis tumour cells were treated with cisplatin (7.5 or 15 μg/ml) for 1 h. The levels of GpG-intrastrand crosslink were determined 6 h post-treatment. D) MGH-U1 bladder, 833 K and SuSa testis and XPA-deficient XP12RO cells were treated with cisplatin (7.5 or 15 μg/ml) for 1 h, GpG-intrastrand crosslink levels were determined 6 and 24 h post-treatment. The levels measured 6 h post-treatment were set to 100%. The levels measured 24 h post-treatment were corrected for dilution due to DNA synthesis during the recovery period. Each point represents the mean of 3 to 6 replicate experiments. E) MGH-U1 bladder (circles) and 833 K testis (squares) tumour cells were treated with cisplatin for 1 h and analysed for ICL cross-linking 7 h post-treatment. The results are the means of three independent experiments. F) MGH-U1 bladder, 833 K and SuSa testis and ERCC1-deficient 43-3B cells were treated with cisplatin (15 μg/ml) for 1 h, the level of ICL cross-linking was determined 7 and 24 h post-treatment. Each column represents the mean of 3 to 6 replicate experiments.