Figure 2
From: Multiple functions of CXCL12 in a syngeneic model of breast cancer
The effect of CXCL12 and CXCL12 (P2G) expression on growth and metastasis of 4T1.2 tumors. A) Effects of CXCL12(P2G) and CXCL12 expression on the formation of spontaneous lung metastases in 4T1.2 tumor-bearing mice. Balb/c mice were injected with 4T1.2, 4T12P2G or 4TX12 cells i.m.f.p.. On day 21, lungs were removed and surface lung metastases were counted under a dissecting microscope. Bars represent the median. *, P < 0.05; ***, P < 0.001 (Kruskal-Wallis), n = 11-21 from two independent experiments. B) BALB/c mice were injected with 4T1.2, 4T12Ala, 4T12P2G or 4TX12 cells i.m.f.p. and tumor growth was monitored using digital calipers. C) At the end of the experiment, mice were killed and their tumors resected and weighed ***, P < 0.0001 (ANOVA), n = 12. Data points and bars represent mean ± SEM. D) Effects of CXCL12(P2G) and CXCL12 expression in 4T1.2 cells on the formation of experimental lung metastases. BALB/c mice were injected intravenously with 7.5 × 105 cells in PBS. After approximately 2 weeks, lungs were harvested and surface metastatic nodules were enumerated under a dissecting microscope. Data points represent the number of surface metastatic nodules of individual mice. Bars represent the median. **, P < 0.01 (Kruskal-Wallis).