Figure 4
From: P-cadherin counteracts myosin II-B function: implications in melanoma progression
Function of myosin II-B in BLM melanoma cell migration and invasion. (A) Western blot to verify silencing of myosin II-B expression 72 h post-transfection in BLM LIE and BLM P-cad cells. Matrigel invasion assay shows reduced invasion of BLM LIE siMyosin II-B when compared to control cells (p < 0,0001). No significant difference could be observed for BLM P-cad siMyosin II-B compared to BLM P-cad siControl (p = 0,2). (B) Reduced migration of BLM LIE siMyosin II-B compared to BLM LIE siControl could be observed (p = 0,008) (upper panel). No significant difference between BLM P-cad siControl and siMyosin II-B could be detected (p = 0,7). Results of a wound healing assay with addition of specific kinase inhibitors (lower panel). KT-5926 significantly reduced cellular migration when compared to control situation, in contrast to Y27632 (p = 0,009; p = 0,98).