Figure 1

Activity of nemorubicin in cells with NER defects. Panel A. Inhibition of colony formation in CHO isogenic cells AA8 with a wt NER (black circle), CHO-UV96, black triangle) lacking the expression of ERCC1, CHO-UV61 (black square) lacking the expression of CSB/ERCC6 and ERA5 (white square) derived from UV96 in which the human ERCC1 gene was transfected and the NER activity restored. Results are reported as percentages of the inhibition relative to untreated cells and are the mean and SD of at least three replicates. Panel B. Activity of nemorubicin in L1210 cells with functional NER (L1210/DDP, black circle ) and NER defective L1210/0 (black square). Results are reported as percentages of the inhibition relative to untreated cells and are the mean and SD of at least three replicates. Panels C and D. Response of L1210 parental cells and of L1210 nemorubicin-resistant cells (L1210/MMDX) to UV (Panel D) and trabectedin (Panel C). Cells were treated with different concentrations of trabectedin or different UV doses and counted 72 hours later. The values are the mean +/- SD of three replicates. L1210 parental cells (black circle), L1210/MMDX cells (black square).