Figure 2
From: Regulation of Erk1/2 activation by osteopontin in PC3 human prostate cancer cells
OPN induces activation of c-Raf and MEK1/2. Protein lysates from PC3 and PC3/OPN cell lines were immunoblotted with phospho-specific antibodies of A-Raf, B-Raf, c-Raf, and MEK1/2 (upper panels in A-D). Immunoblotting with the corresponding non-phosphorylated antibodies (lower panels in A-C) or GAPDH antibody (lower panel in D) was used to demonstrate equal loading. Results shown are representative of three independent experiments.