Figure 2

Effect of ANT2 shRNA on expression of TRAIL receptors in breast cancer cell lines. TRAIL receptor expression in MCF7 and MDA-MB-231 cells (A), MCF7 cells transfected with scrambled or ANT2 shRNA for 24 h (B), and T47 D and BT474 cells transfected with scrambled or ANT2 shRNA for 24 h (C) was analyzed by SDS-PAGE followed by immunoblotting of cell extracts with antibodies against DR4, DR5, DcR1, DcR2, and β-actin (control) as indicated.