Figure 5

Loss of Sin3A increases expression of apoptotic genes differentially in ERα-positive and ERα-negative cell lines. (A) MCF7 or (B) MDA-MB-231 cells were transfected with scrambled (scr.) negative control or Sin3A siRNA, followed by treatment with vehicle control ethanol (EtOH) or 10 nM estrogen (E2) for four hours. qRT-PCR was performed for the indicated apoptotic gene, with ribosomal protein P0 serving as the housekeeping normalization gene. RNA levels were calculated relative to the scrambled ethanol sample. Data are from a minimum of three independent experiments with error bars showing the standard error of the mean. Student's t tests were performed comparing corresponding scrambled and Sin3A siRNA samples, *p < 0.05. (C) Knockdown of Sin3A protein was also confirmed by western blot analysis in each cell line. The blot was reprobed with β-actin for the loading control.