Figure 3
From: Frequent epigenetic inactivation of RASSF2 in thyroid cancer and functional consequences
Methylation of RASSF2 and RASSF5A in primary tissue. A. COBRA of RASSF2 in primary thyroid specimen, including follicular thyroid cancer (FTC), papillary thyroid cancer (PTC), medullary thyroid cancer (MTC), undifferentiated thyroid cancer (UTC), goiter (G) normal thyroid samples (N) and follicular adenoma (FA). PCR products from bisulfite-treated DNA were digested (+) and mock digested (-) with TaqI and resolved on 2% gel. B. Methylation status of RASSF5A. In vitro methylated DNA (methy.) was utilized as positive control. The sizes of a 100 bp ladder (M) are indicated. Methylated (m), partially methylated (p) and unmethylated (u) samples were determined.