Figure 6
From: Frequent epigenetic inactivation of RASSF2 in thyroid cancer and functional consequences
Tumor suppressor function of RASSF2. A. The RASSF2 was cloned in pCMV-Tag1 and transfected in thyroid cancer cell line 1736 in parallel with empty vector in triplicates. Cells were grown in selective medium for four weeks and stained with Giemsa. B. Numbers of colonies were determined and means with standard deviation were plotted. C. 1736 cells were transfected with the indicated fluorescent RASSF2 constructs and control vector (pEYFP). After two days cells were fixed and stained with DAPI. Fluorescent cells (n > 200) were counted and the percentage of intact, condensed, fragmented and missing nuclei were determined. D. Nuclei alterations in FTC133 cells (n > 100).