Figure 3

Triptolide did not affect the 26 S proteasome activity but enhanced the levels of HIF-1α mRNA. A. SKOV-3 cells were treated with triptolide for 12 h under hypoxia condition and then harvested for the detection of 26 S proteasome activity as described in the Materials and Methods. B. The lysates of SKOV-3 cells were incubated in the presence or absence of triptolide and then assayed for the 26 S proteasome activity. C and D. The levels of HIF-1α mRNA from reverse transcription -PCR (C) and real-time PCR (D) analyses in the hypoxic SKOV-3 cells treated with triptolide for 12 h. The levels of HIF-1α mRNA were normalized with β-actin mRNA expression; columns, mean of three independent experiments; bars, SD. The significant difference between triptolide-treated groups and hypoxia-control groups was analyzed by Student t test. * P < 0.05.