Figure 1
From: An essential role for Ran GTPase in epithelial ovarian cancer cell survival
Ran expression in parental cell lines, mixed populations and clones. Comparison of Ran mRNA levels by Q-PCR in TOV112D TetR (a) and TOV1946 TetR (b) parental cell lines, shRNA Ran or LacZ mixed populations and clones. Levels were normalized to parental cell line without induction. Values represent the mean ± SD of two separate experiments each performed in duplicate. Western blot analysis of TOV112D TetR (c) and TOV1946 TetR (d) parental cell lines, mixed populations and clones shRNA Ran or LacZ without and after three days of induction. ß-actin was used as loading control. P: Parental cell line, M.P.: Mixed population, #1, #2 and #3: independent clones. Mann-Whitney test indicates a significant difference with a p-value < 0.05 (*).