Figure 2

Effect of Ran depletion on proliferation. Growth curves for TOV112D TetR (a) and TOV1946 TetR (b) parental cell lines, mixed populations and clones expressing shRNA Ran. Cells were induced with tetracycline three days prior to day 0. Cells were trypsinized and counted every 24h for five days. Values represent mean ± SD of duplicate wells from three independent experiments. Cellular morphology of TOV112D TetR (c) and TOV1946 TetR (d) on day 5 of the growth curve. Loss of Ran GTPase expression results in cell number reduction and round shaped cells. All pictures were taken at 100× magnification. P: Parental cell line, M.P.: Mixed population, #1, #2 and #3: independent clones. Mann-Whitney test indicates a significant difference relative to the non-induced parental cell line with a p-value < 0.05 (*). There was no significant difference between the different non-induced cells.