Figure 3

Loss of Ran expression results in a caspase-3 associated apoptosis in ovarian cancer cell lines. TOV112D TetR (a) and TOV1946 TetR (b) parental cell lines, mixed populations and clones expressing shRNA LacZ and Ran were induced with tetracycline for the indicated times and then fixed in 70% ethanol. Cells were treated with propidium iodide and sub-G₁ peaks were quantified by flow cytometry. Values represent the mean ± SD of three independent experiments. Western blot analysis showing caspase-3 cleavage in cell depleted in Ran expression after 3 days of induction in TOV112D TetR (c) and TOV1946 TetR (d). Kinetic of caspase-3 cleavage by western blot analysis in TOV112D TetR (e) and TOV1946 TetR (f) parental cell lines, mixed populations and clones expressing shRNA LacZ and Ran. TOV112D TetR (g) and TOV1946 TetR (h) parental cell lines, mixed populations and clones expressing shRNA LacZ and Ran were induced with tetracycline for the indicated time and then treated with TMRE for 20 minutes. ΔΨm reduction was measured by flow cytometry. Values represent the mean ± SD of three independent experiments. P: Parental cell line, M.P.: Mixed population, #1, #2 and #3: independent clones. Mann-Whitney test indicates a significant difference relative to the non-induced sample with a p-value < 0.05 (*).