Figure 4

Loss of Ran expression results in tumor regression in vivo. A. TOV112D TetR mixed population and clone expressing shRNA Ran were subcutaneously injected in SCID mice and tumor growth was measured biweekly. All mice developed tumors albeit of varying size and tumor volume. Doxycycline-supplemented food inducing shRNA expression started at day 9. M.P: Mixed population, Ran #2: clone. Values represent mean ± SE of groups of 4 mice. Mann-Whitney test indicates a significant p-value < 0.05 (*). B. Immunohistochemistry analysis of the xenografts on day 6 of the doxycycline-supplemented food showing morphological hematoxylin eosin (HE) staining and Ran, cleaved caspase-3 and Ki67 expression. Slides were obtained from contiguous tissues.