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Figure 2 | Molecular Cancer

Figure 2

From: Synergistic epigenetic reactivation of estrogen receptor-α (ERα) by combined green tea polyphenol and histone deacetylase inhibitor in ERα-negative breast cancer cells

Figure 2

Treatment with EGCG and TSA retrieved responsiveness to E 2 and tamoxifen in ER-negative cells. A) Cellular viability in response to E2 and tamoxifen. B) The expression of PGR, an ERα target gene, in response to E2 and tamoxifen. EGCG and/or TSA-pretreated MDA-MB-231 cells were treated with or without 10 nM of E2 or 1 μM tamoxifen for 2 days. MCF-7 cells served as a positive control. Cells were harvested at the indicated time periods and assessed for cellular viability and PGR expression, respectively. Cellular viability was measured by Trypan blue exclusion assay. PGR expression was detected by quantitative real-time PCR. Data were obtained from three independent experiments and normalized to GAPDH and calibrated to levels in samples without treatment of E2 and tamoxifen. Bars, SD; *, P < 0.05, significantly different from control.

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Molecular Cancer

ISSN: 1476-4598