Figure 3
DHPCC-9 decreases migration of prostate cancer cells without affecting Pim protein levels or cell viability. PC-3 prostate cancer cells were treated with DMSO or 10 ÎĽM DHPCC-9. Two hours later, scratch wounds were made with a 10 ÎĽl sterile pipette tip. (A) Representative pictures were taken at indicated time-points and analysed. (B) Graph represents means from triplicate samples after 24 h incubation. (C) Endogenous Pim expression levels were determined by Western blotting. GAPDH staining was used as a loading control. (D) Cell viability was analysed by the MTT assay. (E) The amount of live cells was determined by Trypan blue staining.