Figure 4
DHPCC-9 also reduces migration of squamocellular carcinoma cells. UT-SCC-12-A squamocellular carcinoma cells were treated with DMSO or 10 ÎĽM DHPCC-9. Two hours later, scratch wounds were made with a 10 ÎĽl sterile pipette tip. (A) Representative pictures were taken at indicated time-points and analysed. (B) Graph represents means from triplicate samples. (C) Endogenous Pim expression levels were determined by Western blotting. GAPDH staining was used as a loading control. (D) Cell viability was analysed by the MTT assay.