Figure 2

BRG1 modulates the expression of extracellular matrix and adhesion molecule expression in SK-MEL5 cells. The expression of extracellular and adhesion molecule related genes was profiled in control SK-MEL5 (empty vector) and BRG1 reconstituted SK-MEL5 cells using a focused qPCR array. The expression level of each gene was normalized to a control value obtained by averaging the expression of four housekeeping genes. The average of three to four independent experiments is shown. All changes in gene expression are statistically significant as determined by the student's t test (p value equal to or less than 0.05). A. Expression of genes that are activated greater than four-fold by BRG1. B. Expression of genes that are activated greater than two fold by BRG1. C. Expression of genes that are down-regulated by BRG1. D. MCAM expression was evaluated separately from the qPCR array using gene specific primers. Normalization of mRNA levels was to GAPDH. E. Detection of NCAM1 and MCAM protein expression by Western blotting. Tubulin is a loading control.