Figure 4
BRG1 expression alters adhesion of SK-MEL5 cells to different ECM components. 96 well plates were coated with laminin, collagen, or fibronectin and blocked with BSA. Control SK-MEL5 cells expressing empty vector (EV) and SK-MEL5 cells expressing BRG1 were seeded onto coated plates at a density of 2 × 104 cells. After incubation for 30 minutes, plates were washed and the cells stained with crystal violet. Control plates coated with BSA were also included but did not retain cells after washing (data not shown). Representative fields are shown (20× magnification). Adhesion was quantified by reading absorbance at 550 nm. The data shown is the average of two independent experiments done in triplicate. The fold change in adhesion to all three substrates was significantly altered by expression of BRG1 (p < 0.01). A. Adhesion to laminin. B. Adhesion to collagen. C. Adhesion to fibronectin.