Skip to main content
Figure 7 | Molecular Cancer

Figure 7

From: Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness

Figure 7

SP1 interacts with BRG1 to regulate MMP2 expression. A. SK-MEL5 +BRG1 cells were transfected with Acell SMART Pool siRNAs targeting SP1 or red non-targeting siRNAs. SP1 expression was analyzed by Western blotting 144 hours after transfection. B. MMP2 secretion in control and SP1 down-regulated cells was analyzed by zymography 144 hours after transfection. C. Co-immunoprecipitations were performed with FLAG antibody or control IgG. Co-immunoprecipitating proteins were analyzed by Western blotting with the indicated antibodies. D. Chromatin immunoprecipitations (ChIPs) were performed with antibodies to SP1, BRG1, or control IgG and analyzed by qPCR with primers specific for the MMP2 promoter or a control CD25 region. The results from two independent experiments were assayed twice. BRG1 was significantly enriched on the MMP2 promoter compared to the control CD25 region (p < 0.01). SP1 binding to the MMP2 promoter was significantly increased in SK+MEL5+BRG1 compared to control cells (p < 0.01).

Back to article page

Molecular Cancer

ISSN: 1476-4598