Figure 1

Effects of C3G on ethanol-mediated invasion/migration of breast cancer cells. A: MCF7^ErbB2 cells were plated into the upper compartments of the matrigel invasion chambers and exposed to ethanol (0 or 400 mg/dl) with/without C3G (10 μM) for 48 h. Following the treatment, the invasive potential was assayed as described under the Materials and Methods and presented relative to untreated controls. B: MCF7^ErbB2 cells were exposed to ethanol (0, 200 or 400 mg/dl) with/without C3G (10 or 20 μM) for 48 h. The invasive potential was assayed as described above. C: MCF7^ErbB2 cells were plated into the upper compartments of the migration chamber and exposed to ethanol (0 or 400 mg/dl) with/without C3G (10, 20 or 40 μM) for 12 h. The migration was analyzed as described under the Materials and Methods and presented relative to untreated controls. D: BT474, MDA-MB231 or MCF7^ErbB2 cells were exposed to ethanol (0 or 400 mg/dl) with/without C3G (10, 20 or 40 μM) for 48 h. Their invasion potential was evaluated as described above. E: BT474, MDA-MB231 or MCF7^ErbB2 cells were exposed to ethanol (0 or 400 mg/dl) with/without C3G (10, 20 or 40 μM) for 48 h and cell viability was determined with MTT assay. The number of viable cells was presented relative to untreated controls. Each datum point was the mean ± SEM of three independent experiments. * denotes a statistically significant difference from untreated controls. # denotes a significant difference from ethanol-treated groups. ε denotes a significant difference from ethanol- and C3G (10 μM)-treated groups. δ denotes a significant difference from ethanol- and C3G (20 μM)-treated groups. F: MDA-MB231 cells were exposed to ethanol (0 or 400 mg/dl) with/without C3G (10 μM) for 24 h and cell migration was determined by wound healing migration assay as described under the Materials and Methods.