Effects of C3G and antioxidants on ethanol-induced ROS generation, cell invasion and ErbB2 phosphorylation. A: MCF7ErbB2 cells were exposed to ethanol (0 or 400 mg/dl) with/without C3G (10 μM), SOD (50 U/ml)/catalase (200 U/ml), NAC (5 mM) or vitamin C (20 μM) for 48 h. Intracellular ROS levels were measured by flow cytometry as described under the Materials and Methods. B: The invasive potential of MCF7ErbB2 cells was evaluated as described above and expressed relative to untreated controls. C: The phosphorylation of ErbB2 in MCF7ErbB2 cells was analyzed with immunoblotting. The experiment was replicated three times. * denotes a statistically significant difference from untreated controls. # denotes a significant difference from ethanol-treated groups. # denotes a significant difference from ethanol- and C3G-treated groups.