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Figure 3 | Molecular Cancer

Figure 3

From: Podoplanin in cancer cells is experimentally able to attenuate prolymphangiogenic and lymphogenous metastatic potentials of lung squamoid cancer cells

Figure 3

Suppressive effect of tumor cell-associated podoplanin on tumor lymphangiogenesis. A) Immunohistochemical staining for human podoplanin with EBC1-P4-derived tumor sections. Representative results are indicated. Right photograph is a magnification of the box shown in the left photograph. Podoplanin expression was observed at the cell surface (arrows). B) Implanted tumor tissues were subjected to HE staining and immunohistochemical staining for mouse LYVE-1. Representative photographs (for EBC1-V1 and EBC1-P4) are indicated. LYVE-1-positive signals (arrows) were observed. C) Serially sectioned tumor tissues derived from EBC1-V1 were subjected to immunohistochemical staining for mouse LYVE-1 (upper photograph) and to Masson staining (lower photograph). Tumor cell nests surrounded by podoplanin-positive lines (arrows) showed a negative reaction to Masson staining (arrowheads). D) The area, the perimeter, and the number of LYVE-1-positive lymphatic vessels were measured and evaluated as described in Methods (n = 3 each, *p < 0.001 versus V1).

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Molecular Cancer

ISSN: 1476-4598