RGS10 and RGS17 knock-down suppresses cisplatin-induced cell death. (A) SKOV-3 cells were transiently transfected with control or RGS-targeted siRNA constructs and treated with vehicle or 10 μM cisplatin for 48 hours. Cells were then fixed and stained with annexin V-FITC to quantify externalized phosphatidyl serine. Cells were analyzed by flow cytometry, and gated to calculate percentage of cells staining positively for annexin. In each plot, PI staining intensity is indicated on the y-axis and annexin staining intensity is indicated on the x-axis. Cells to the bottom and right of the indicated diagonal line were scored as annexin positive. (B) The percentage of annexin-positive cells was determined at each siRNA transfection condition in the presence of increasing doses of cisplatin. 20,000 cells were analyzed for each data point.