Figure 4

Western blotting analysis of total β-catenin (Anti-β-catenin) and active nonphosphorylated β-catenin (Anti-active β-catenin). A, the five parathyroid carcinomas. Mean methylation levels (Additional file 1, Table S1) of the APC promoter 1A are also shown. B, normal parathyroid tissue specimens (n = 5). C, active β-catenin level in cultured primary PC1 cells treated with 5-aza-2'-deoxycytidine for the indicated time points.