Figure 4

hMSCs enhance SDF-1 gene transcription in vivo and in vitro. (A) MCF-7 cells were treated with conditioned media from MCF-7 cells (control) or hMSCs for 24 hours. Cells were then harvested for PCR analysis of CXCR4 and SDF-1. (B) Endpoint oestrogen and matrigel tumours were harvested at day 36 post cell injection and gene expression analyzed by qPCR. (C) Endpoint matrigel tumours grown in the absence of oestrogen were harvested at day 44 and gene expression analyzed by qPCR. Data represent fold change relative to control as normalized to internal β-actin ± SEM, (*, p < 0.05).