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Figure 3 | Molecular Cancer

Figure 3

From: Human homolog of Drosophila H airy and e nhancer of s plit 1, Hes1, negatively regulates δ-catenin (CTNND2) expression in cooperation with E2F1 in prostate cancer

Figure 3

RT-PCR and real-time qRT-PCR analyses of mRNA expression of δ-catenin and its potential transcriptional regulators. (A) RT-PCR. Total RNAs were prepared from human prostate cancer cell lines (PC3 and CWR22-Rv1) and a non-cancer prostate cell line (PZ-HPV-7). RT-PCR analyses were performed to determine the mRNA expression of Hes1, Hey1, E2F1, Pax6, p53, AR, and δ-catenin genes. GAPDH was used as control. Numbers beneath each lane are the semi-quantification of RT-PCR data normalizing to GAPDH. (p < 0.05) (B) Real-time qRT-PCR analyses of relative mRNA levels of E2F1, Hes1, and δ-catenin in comparison to GAPDH. While the trends were clear that the transcript levels of E2F1 and Hes1 were higher in PC3 cells than that in CWR22-Rv1 cells, they were not statistically significant. Three independent experiments were performed.

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Molecular Cancer

ISSN: 1476-4598