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Figure 8 | Molecular Cancer

Figure 8

From: Human homolog of Drosophila H airy and e nhancer of s plit 1, Hes1, negatively regulates δ-catenin (CTNND2) expression in cooperation with E2F1 in prostate cancer

Figure 8

Expression of transcriptional regulators of δ-catenin in NE-10 mice bearing prostate tumors. (A) Left panel: RT-PCR of δ-catenin, Hes1 and E2F1 gene expressions in one representative set of normal prostate (10 week old CD1 mouse) and NE-10 tumor tissues [14]; Right panel: Immunohistochemistry showing increased δ-catenin expression in NE-10 mouse tumor specimen as compared to a normal mouse prostate. a. Normal mouse prostate tissue. b. NE-10 tumor tissue. Original magnification: x400. (B) Comparison of real-time qRT-PCR of δ-catenin, Hes1, and E2F1 gene expression between normal prostate and NE-10 tumors (GADPH as control). While δ-catenin transcript level was consistently increased, Hes1 expression was diminished. However, E2F1 transcript level varied greatly. (C) Comparison of real-time qRT-PCR of Hes1 and E2F1 gene expression among NE-10 tumors. Among the 11 NE-10 tumors, E2F1 transcription was relatively high when Hes1 level was correspondingly high. When the E2F1 transcription was relatively low, Hes1 expression was extremely low. These experiments were repeated twice, and the results were similar.

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Molecular Cancer

ISSN: 1476-4598