Figure 3

H. pylori induces nuclear β-catenin accumulation LEF/TCF activation and target gene expression. A, NCI-N87 cells were infected for 1 h with H. pylori P1 wildtype strain or treated with recombinant Wnt3a or heat-killed bacteria (hk) for 1 h, respectively. Nuclear fractions were prepared (S-PEK Kit, Calbiochem). Western blot analysis was performed with antibodies as indicated. B, Cells were transiently transfected with either 8xSuperTOPflash or FOPflash and cotransfected with renilla luciferase plasmid. 24 h later, the cells were infected with H. pylori P1 wildtype strain or treated with recombinant Wnt3a, lysed and firefly/renilla luciferase activity was measured. Diagram shows the mean fold changes + SEM of 3 separate experiments performed in triplicate. * p < 0.01, relative to activity in non-infected cells. C, NCI-N87 cells were infected for 3 h with H. pylori P1 wildtype strain or treated with recombinant Wnt3a or heat-killed bacteria for 3 h. Cytosolic fractions were prepared (S-PEK Kit, Calbiochem). Western blot analysis was performed with the indicated antibodies.