Figure 3

Suppression of miR-342 promotes tamoxifen resistance. The tamoxifen sensitive MCF-7/pcDNA and MCF-7/HER2 cell lines cultured for 48 hr in 5% CS-FBS MEM were transfected with anti-miR-342 or anti-miR negative control (NC) and the next day treated with 100 pM E2 alone or in combination with 1.0 μM TAM for five days. (A) MTT assay was used to quantitate cell growth and (B) apoptosis was quantitated using a Cell Death Detection ELISA. Data is represented as mean +/- SE of three independent experiments relative E2 treated MCF-7/pcDNA cells. Asterisks indicate samples with significant differences as determined by paired Student's t test (A, p < 0.01; B, p < 0.005).