Ectopic overexpression of FBI-1 promoted motility, invasiveness and proliferation of ovarian cancer cells. A, In vitro migration (upper panel) and invasion assays (lower panel) in OVCA 420 (wild-type p53) and SKOV-3 (null p53) cell lines after transfection of pEGFP-FBI-1. B, mRNA expression of FBI-1, MT1-MMP, MMP-2 and TIMP-2 in cells after transfection of pEGFP-FBI-1 (upper panel). Western blot analysis of the expression of exogenous GFP-tagged FBI-1 (GFP-FBI-1) and endogenous FBI-1 (Endo-FBI-1) (as detected by anti-FBI-1 antibody) and MT1-MMP in cells after transfection of pEGFP-FBI-1 (lower panel). C, In vitro migration (upper panel) and invasion (middle panel) assays in OVCA 420 and SKOV-3 cells transiently transfected with pEGFP-FBI-1, or control vector combined with siRNAs of MT1-MMP or control. Western blot analysis (lower panel) of the expression of GFP-FBI-1 and Endo-FBI-1, and MT1-MMP in cells expressing FBI-1 transfected with siRNA of MT1-MMP. D, MTT assays revealed that overexpressed FBI-1 promoted cell growth of OVCA 420 which possessed wild-type p53, but not SKOV-3 cells with mutation and loss of expression of p53.