Figure 2
From: EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis
EBER1 decreased p21cip1/waf1 transcription in HL cell lines. 2A: Transcript profiles of p21cip1/waf1-related genes. The z-scores, ZK in white and ZL in black, were plotted for the following genes: 1) p21cip1/waf1 & its regulators: EGR1 and STAT1, 2) cell cycle-related genes: p53 and cyclins. Note that the Affymetrix chip used multiple sets of probes to monitor a single gene, but only EGR1 and STAT1 showed consistent decreases across all probe sets. 2B: Eight splicing variants of p21cip1/waf1 have been reported: variant 1, variant 2, Alternative-a (Alt-a), Alternative-a' (Alt-a'), Alternative-b (Alt-b), Alternative-c (Alt-c), B, and C. EBER1 did not change the splicing patterns, as RT-PCR showed that variant 1 was always the dominant form for K9, KE, L9, and LE. X-axis: p21 variants; Y-axis: proportions of the variants. Only the major variants, variant 1, variant 2, B, and Alt-a were shown. The others variants were all less than 1%. 2C: Representative real-time RT-PCRs for p21cip1/waf1 were shown. X-axis: cDNA amount; Y-axis: dCT (the threshold cycle of p21cip1/waf1 minus that of actin). Triplicate measurements were taken at each one of 4 cDNA concentrations. The dCT (mean +/- SD) was 6.2 +/- 0.2 for K9, 8.7 +/- 0.4 for KE, 11.5 +/- 0.2 for L9, and 13.0 +/- 0.4 for LE.