Figure 3
From: EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis
EBER1 suppressed p21cip1/waf1 without changes in cell cycle distributions. 3A: EBER1 suppressed p21cip1/waf1. Left panel: EBER1 decreased p21cip1/waf1 in KE and LE, although overloading was necessary to show the much weaker levels of p21cip1/waf1 in L9 & LE. Right panel: EBER1 but not antisense EBER1 suppressed p21cip1/waf1. 3B: EBER1 suppressed EGR1, STAT1, and p53, but had no consistent effects on SirT5. The doublets of STAT1 were due to isoforms. 3C: Western blotting showed decreased cyclin D2 in KE & LE, but no changes in the levels of CDKs or other cyclins. 3D: Cell cycle distributions of K9 (dash) and KE (solid) on the left, L9 (dash) and LE (solid) on the right. There were no detectable differences in the cell cycle distributions between KE & K9, or LE & L9. The Y-axis is the number of cells. The X-axis is the intensity of propidium iodide (PI) on a linear scale from zero to 1000. The first peak at around 250 had diploid DNA content (2N) and was the peak of the G0/G1 phase. The second peak at around 500 had quadruploid DNA content (4N) and was the peak of the G2/M phase. Between these 2 peaks, cells were in S phase.