Figure 4
From: EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis
EBER1 conferred resistance to apoptosis induced by TSA & MG115. 4A & 4B: Left panel: Apoptosis induced by 0.5 μM TSA (4A) or 0.4 μM MG115 (4B) for 2 days was measured by flow cytometry. The X-axis is Annexin-V (AV) and the Y-axis is propidium iodide (PI). Both the X and Y-axes are in log scale from 100 to 104. Green: AV-/PI- viable cells. Blue: AV+/PIdim apoptotic cells. Red: AV+/PIbright dead cells. Right panel: The percentages of viable cells after treatments were measured. (Y-axis: percentage of viable cells; X-axis: cell lines). Note that L9 was the most sensitive cell line after TSA treatment (4A), and K9 was the most sensitive cell line after MG115 treatment (4B). 4C & 4D: Induction of p21cip1/waf1 by 0.5 μM TSA (4C) or by 0.4 μM MG115 (4D) was measured with ELISA. (Y-axis: p21cip1/waf1 of treated cell lines divided by that of untreated cell lines; X-axis: cell lines). Note that L9 showed the highest induction after TSA treatment and K9 showed the highest induction after MG115 treatment. 4E & 4F: After treatment with 0.5 μM TSA, L9 cells grew slower than LE cells(4E), and after treatment with 0.4 μM MG115, K9 cells grew slower than KE cells (4F). (Y-axis: cell numbers in 104; X-axis: duration of culture in days; UNT: untreated).