Figure 4

The effect of pentamidine on DNA double-strand break repair (A)(B) Pentamidine decreased homologous recombination activity in DR-GFP assay. I-SceI expression plasmids were introduced to M5D (A) or HeLa-DRGFP cells (B) by an electroporation with or without pre-treatment of pentamidine (0.5 mM, 1 hour). After 3 days GFP-positive cells, induced via HR pathway, were analyzed by flowcytometer as described in Materials and Methods. (C) Pentamidine did not disturb NHEJ activity. I-SceI expression plasmids were introduced to MRC5-pEJ cells by electroporation with or without pre-treatment of pentamidine (0.5 mM, 1 hour). After 3 days GFP-positive cells, induced through NHEJ pathway, were analyzed by flowcytometer as described in Materials and Methods.