Figure 1

Double immunohistochemical staining for CD8/Foxp3, Foxp3/GrB and Foxp3/IL-17. A. NPC tumor tissue with numerous CD8⁺ (red) and Foxp3⁺ (brown) cells (× 200). B. NPC tumor tissue with numerous Foxp3⁺ (brown) and GrB⁺ (red) cells (× 200). C. NPC tumor tissue with numerous Foxp3⁺ (red) and IL-17⁺ (brown) cells (× 200). The staining patterns show that CD8 is on the cell membrane, GrB and IL-17 are in the cytoplasm, and Foxp3 is in the nucleus. There are a few CD8⁺Foxp3⁺ and Foxp3⁺GrB⁺ cells, but no Foxp3⁺IL-17⁺ cells.