Figure 1

Expression of CD7 and ELA2 is associated with DNA hypomethylation in leukemic cell lines. Bisulfite sequencing and CoBRA for A) ELA2, on THP-1 (positive) and RPM1 (negative); and B) CD7 on THP-1 (negative) and ALL-SIL (positive). Cartoons show gene organization, with transcriptional start sites as bent arrow, UTR as narrow bars and translated regions as wide bars. Down arrows show location of enzyme sites used for CoBRA. Vertical bars show location of CpG dinucleotides. Dotted lines show location of region amplified after ChIP. Open and filled circles indicate unmethylated and methylated CpGs respectively. Bottom panels show CoBRA for each gene. Enzymes used are indicated as follows; U = uncut, H = Hin fI, R = Rsa, T = Taq Iα and M = Mbo I. Position of the uncut band is indicated by an arrow.