Figure 5

AZD1152 inhibits growth of lung tumor nodules in a lung metastasis nude mouse model. (A) Two million MDA-MB-231 human breast cancer cells were injected via tail veins of nude mice. AZD1152 (125 mg/kg/dose) or vehicle were administered IP on days 1 and 2 of 7-day repeating cycle for 4 cycles starting 2 days after cancer cell injection. Ten weeks after cancer cell injection, the mice were sacrificed and lungs were weighed and plotted as the means for each treatment group. (B) Matched lobes of the lungs from a representative mouse from each group are shown as labeled. (C) The number of macroscopic tumor nodules in the lungs was counted in each mouse. The mean number of nodules per mouse was plotted for each group. Error bars represent 95% confidence intervals. (D) H&E stained slides of lungs from control and drug treated mice are shown at the same magnification. Arrows indicate metastatic lung tumors.