Figure 5

PGE ₂ induces α2β1 integrin upregulation and cell migration through NF-κB activation. Cells were pretreated with PDTC or TPCK for 30 min, then they were followed by stimulation with PGE₂, and in vitro migration (A) and α2β1 integrin expression (B) were measured with the Transwell and flow cytometry after 24 hr. (C) JJ012 cells were incubated with PGE₂ for indicated time intervals, p-IKK, p-IκBα and p-p65 expression was determined by Western blot analysis. (D) Cells were transfected with IKKα or IKKβ mutant for 24 hr followed by stimulation with PGE₂, and in vitro migration measured with the Transwell after 24 hr. (E) Cells were pretreated with U73122, GF109203X, PP2, PDTC, TPCK, PDTC for 30 min or co-transfected with PLCβ siRNA, PKCα mutant, IKKα mutant, IKKβ mutant for 24 hr before incubation with PGE₂ for 24 hr. The NF-κB activity was measured. (F) Cells were pretreated with U73122, GF109203X or PP2 for 30 min followed stimulation with PGE₂ for 60 min, and p-p65 expression was examined by Western blot analysis. Results are expressed as the mean ± S.E. *, p < 0.05 compared with control; #, p < 0.05 compared with PGE₂-treated group