Figure 3
Effect of SFN on DNA synthesis of MDAH-2774 cells. A. Cells were seeded at 106 cells and grown serum-free medium for 24 hr for cell cycle synchronization. Cells were then treated with either vehicle DMSO (i, ii and iii) or 10 μM SFN (iv, v and vi) for 0 hours (i and iv), 24 hours (ii and v) and 48 hours (iii and vi), respectively. Cells were fixed, stained and flow cytometric analysis was conducted using a cell cycle phase determination kit (Cayman chemical company, Ann Arbor, MI). B. Tabular representation of the % changes in the cell cycle phases. * Metaphase not reported.