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Figure 5 | Molecular Cancer

Figure 5

From: Sulforaphane induces cell cycle arrest by protecting RB-E2F-1 complex in epithelial ovarian cancer cells

Figure 5

Protein expression analysis of cell cycle regulatory proteins RB-E2F-1 interactions: A. Approximately 10 μg of protein extracts of control and SFN treated MDAH-2774 cells were resolved by SDS-PAGE, transferred to nitrocellulose membrane; and probed with E2F-1, CDK-4 and CDK-6 as indicated. β actin was used as a loading control. B. Western blot analysis of SFN treated MDAH-2774 cells (upper panel). SFN treated MDAH-2774 cell lysates were immuno-precipitated with E2F-1 polyclonal antibody and probed with RB monoclonal antibody (Lower panel). P-RB: hyper phosphorylated retinoblastoma protein; RB: Under phosphorylated retinoblastoma protein.

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Molecular Cancer

ISSN: 1476-4598