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Figure 6 | Molecular Cancer

Figure 6

From: CD40 ligand induced cytotoxicity in carcinoma cells is enhanced by inhibition of metalloproteinase cleavage and delivery via a conditionally-replicating adenovirus

Figure 6

Delivery of membrane-bound CD40L via a conditionally replicating adenovirus. (A) Expression of ncCD40L by E1AD24ncCD40L. A549 cells were infected with 5 MOI E1AD24ncCD40L or E1AD24GFP or left uninfected for 48 hours. In parallel, cell were infected with RAdncCD40L (5 or 100 MOI). 10 μg of total protein lysates were blotted for ncCD40L. (B) Analysis of CD40 expression. Total protein lysates from EJ, Hela and A549 cells were harvested and CD40 expression was examined by western blot. β-actin was used as a loading control. (C) Effect of E1AD24ncCD40L on cell viability. EJ, Hela and A549 cells were infected with 5 MOI of either E1AD24GFP (E1AGFP) or E1AD24ncCD40L (E1AncL) or left without infection for 36 hours. Cell viability was then assessed by WST-1 assay. Results are the mean of triplet samples +/- SD. Two-tailed t-test E1A-GFP vs. E1A-ncL: EJ, p < 0.0001; HeLa, p = 0.2879; A549, p = 0.0460.

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Molecular Cancer

ISSN: 1476-4598