Analysis of proliferation, programmed cell death and IGF-I signaling in P5 cerebellum of Ptc1+/+, Ptc1+/-, Ptc1+/+/IGF-I Tg and Ptc1+/-/IGF-I Tg mice. (A) Representative image of Ki-67 and (B) PCNA immunostaining. (C) Graphic representation of frequency of Ki-67, and (D) PCNA positive cells in the EGL. (E) Immunoblot analysis of expression of cyclin D1 with relative β-actin to control protein loading, and (F) relative graphic representation of densitometric analysis. (G) Representative image of caspase-3 positive cells in the EGL. (H) Graphic representation showing the frequency of caspase-3 positive cells in the EGL.