Downregulation of Wnt-11 leads to apoptosis in prostate cancer cells. (a) Extracts from control shRNA and WNT11 shRNA LNCaP cells grown in androgen-depleted medium for 7 days were probed by western blotting for cleaved PARP, cleaved caspase-9 and HSP60. (b) Caspase assays from control shRNA and WNT11 shRNA LNCaP cells grown in androgen-depleted medium for 4 days. Caspase activity was significantly higher in WNT11 shRNA cells (shaded bars) compared to in control shRNA cells (n = 6; p < 0.05). (c) Caspase assays from PC3 cells transfected with control siRNA (white bars) or WNT11 siRNA (shaded bars). Caspase activity was significantly increased in WNT11 siRNA transfected cells at 2 days and 3 days (n = 4; p < 0.001). All data are shown as means ± standard deviation.