Figure 4

In vitro wound-healing assay uncover a selective role for both RHOA and PRKCZ in S2-CP9. Only the combined effect of 50 μM RHOA and 50 μM PRKCZ peptides (as indicated on the left) are effective in inhibiting wound healing selectively in S2-CP9 clone. S2m appear incapable to cover the wound, demonstrating a defect in random movement, therefore the effect of the inhibition of the indicated pathways can not be evaluated in S2m. Photomicrographs at 10× magnification were taken at the beginning and after 6 hours from the start of treatment to assess cell migration and were mounted together following appropriate reference marks. One representative of 3 individual experiments.