Figure 3

Immunohistochemical analysis indicated that overexpression of PGAM1 in HCCs was closely associated with the degree of differentiation and the lower survival rates. (A), staining against PGAM1 confirmed significant differences between normal liver tissues and hepatocellular carcinoma samples more likely to present poor differentiation (p < 0.05). a, normal liver tissue; b, well differentiated tissue; c, moderate differentiation; d, poor differentiation. a-d, representative HE staining; a'-d', expression of PGAM1 was examined by immunohistochemistry. a"-d", representative insets of a'-d'. Original magnification, × 200. (B), Kaplan-Meier survival curves showed the correlation between higher levels of PGAM1 expression and lower survival rates (p < 0.05).