Figure 5From: Hedgehog/Gli supports androgen signaling in androgen deprived and androgen independent prostate cancer cellsCo-immunoprecipitation of AR with Gli2 protein. Lysates of cells (293FT) transfected with AR, myc-tagged Gli2 (Myc-Gli2ΔN) or both for 48 hrs (under androgen-supplemented [R1881] or depleted [CS-FBS] conditions) were immunoprecipitated with α-AR or α-myc antibody. IPs or lysates were electrophoresed and blotted. The Western blot (WB) was probed with α-AR or α-myc antibody as indicated.Back to article page